MSBIO

GelViewer Nucleic Acid Gel Stain, 10,000 × in water

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Catalog Number	Size	Price	Quantity
FLD0703	0.5 mL	￥329.0
FLD0703-1	1 mL	￥589.0

GelViewer Nucleic Acid Gel Stain, 10,000× in water | FLD0703 | 0.5 mL

￥329.0

GelViewer Nucleic Acid Gel Stain, 10,000× in water | FLD0703-1 | 1 mL

￥589.0

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Product Description

GelViewer is a sensitive, stable, low toxicity fluorescent nucleic acid dye for staining dsDNA, ssDNA and RNA in agarose or polyacrylamide gels. It is a safer and more effective alternative to ethidium bromide (EtBr). GelViewer provides excellent band visualization under both UV transilluminator and blue-light imagers, such as blue LED lightboxes. Using blue light helps prevent DNA damage and protect users from UV exposure. GelViewer support both in-gel staining (precast method) and post-staining, and is fully compatible with downstream applications, including gel purification, restriction digest, sequencing, and cloning.

Staining Results

PCR products (1050 bp) stained with GelViewer and imaged using a blue-light transilluminator.

Post-staining of a 1 % agarose gel with GelViewer. Two-fold serial dilutions of λ-DNA/Hind III digest were loaded in amounts of 200, 100, 50, 25, 12.5, 6.25 and 3.125 ng from left to right.

Spectral Profile

Excitation and emission spectra of dsDNA-bound GelViewer nucleic acid gel stain in TBE Buffer

Fig. Excitation and emission spectra of GelViewer nucleic acid gel stain

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Documents

User Manual: GelViewer Nucleic Acid Gel Stain

使用说明: GelViewer 核酸凝胶染料

Troubleshooting

Issue	Solution
Smearing or trailing of DNA bands in pre-stained gels	1. Reduce the DNA loading amount to 1/2 or 1/3 of the original. GelViewer is significantly more sensitive than ethidium bromide (EtBr), and overloading may cause overexposure or smearing of bands.
	2. Consider switching to post-staining instead of pre-staining. Post-staining minimizes interference with DNA migration and improves imaging quality.
	3. For high molecular weight DNA, use a lower percentage agarose gel. Smearing is more common with large DNA fragments.
	4. Use TBE buffer instead of TAE. TBE provides stronger buffering capacity and is better suited for high-resolution electrophoresis.
	5. Avoid using loading buffers containing SDS, as SDS can disrupt dye–DNA binding and alter migration patterns. If SDS is required, post-staining is strongly recommended for optimal results.
Altered DNA migration in pre-stained gels	1. Reduce the DNA loading amount to 1/2 or 1/3 of the original.
	2. Lower the dye concentration; a 0.5× working concentration is recommended for in-gel staining.
	3. Use post-staining instead of pre-staining to minimize interference with DNA migration and improve band resolution and imaging quality.
Reduced fluorescence intensity	1. The dye may have precipitated. Warm the solution at 40–50 °C for 1–2 minutes and vortex thoroughly to redissolve.
Reduced fluorescence intensity	2. Store the dye at room temperature and protect it from light to prevent precipitation caused by low temperatures.

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