TSA is a tyramide-based signal amplification technology for detection of low-level expression of targets like proteins, nucleic acids, which could enhance sensitivity to 50-100 times higher than traditional IHC methods. MSBIO combines high purity of MaxFluor and MaxSulf dye with TSA technology and creates high sensitivity of Tyramide LUMO Kit to help you acquire reliable detection and imaging for low-abundance targets.

After the combination of HRP-labeled secondary antibody with primary antibody, tyramide groups of fluorescent dyes in the proximity will be active, then activated fluorescent dye compounds could covalently combine with aromatic residues (tyrosine, tryptophan, phenylalanine) and precipitate around the target. The precipitation of large amount of fluorescent dye will produce high density of fluorescent dye labeling area to amplifying signal of low-abundance targets. TSA has a wide application view, including Enzyme-linked immunosorbent assay (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence in-situ hybridization, FISH.