EasyView Nucleic Acid Gel Stain, 100 ×
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| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| FLD0704 | 25 mL | ￥600.0 |
EasyView Nucleic Acid Gel Stain, 100 × | FLD0704
| 25 mL
￥600.0
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Overview
EasyView is a next-generation visible-light nucleic acid gel stain independently developed by MSBIO. It enables clear visualization without ultraviolet (UV) illumination, with electrophoresis bands visible to the naked eye. This eliminates UV-related damage to both laboratory personnel and nucleic acid samples, enhances experimental safety, and supports UV-free gel excision and recovery. EasyView offers excellent sensitivity, with a DNA detection limit of ~0.5 ng, comparable to GelRed, and is compatible with downstream applications such as molecular cloning, restriction enzyme digestion, and sequencing.
Components
| Contents | ||
|---|---|---|
| Code.No. | Component | Specification |
| FLD0704 | EasyView Nucleic Acid Gel Stain, 100 × | 25 mL |
Gallery
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Documents
Troubleshooting
| Issue | Solution |
|---|---|
| 染色后看不到DNA条带 | 1. 确保已进行光照显色。EasyView染色后初始无色，需经过明亮光源照射后才能转为蓝紫色。 |
| 2. 提高光照强度或缩短光源距离。光源较弱（如台灯、手机灯）可能需要30–90 min才能显色，建议使用高亮LED并尽量靠近凝胶。 | |
| 3. 延长EasyView染料染色时间，必要时可避光过夜染色。 | |
| 4. 确认染料完全溶解并充分混匀。 | |
| 5. 更换不含蓝色示踪剂的上样缓冲液。含溴酚蓝等蓝色示踪剂的上样缓冲液会遮挡显色的蓝紫色条带。 | |
| 条带显色非常浅或不均一 | 1. 使用高亮LED并缩短光源距离。 |
| 2. 延长EasyView染料染色时间，染色深度受凝胶厚度和胶浓度影响。 | |
| 3. 确保染料完全混匀并按正确比例稀释。 | |
| 背景偏高或出现非特异紫色区域 | 1. 缩短EasyView染料染色时间。 |
| 2. 确认染料正确稀释至1 ×工作浓度。 | |
| 3. 显色期间保持凝胶浸泡在染色液中。凝胶直接暴露在空气中可能局部干燥，导致局部颜色加深或出现斑块状显色。 | |
| DNA条带迁移异常 | EasyView仅适用于后染法。若将染料添加到凝胶或样品中，会干扰DNA的正常迁移，导致条带位置异常。 |
| 显色后DNA条带逐渐变浅或消失 | 1. 将凝胶存放于缓冲液中或密封保存。避免长时间暴露在空气中，以防条带逐渐褪色。 |
| 2. 避免显色后持续强光照射。过度强光暴露可能加速条带褪色。 |