5-Plex Tyramide Lumo Kit
| 货号 | 二抗 | 规格 | 价格 | 数量 |
|---|---|---|---|---|
| TLK0503-50R | Anti-Rabbit lgG | 50 slides | ￥4080.0 | |
| TLK0503-50C | Anti-Mouse and Rabbit lgG | 50 slides | ￥4080.0 | |
| TLK0603-100R | Anti-Rabbit lgG | 100 slides | ￥7680.0 | |
| TLK0603-100C | Anti-Mouse and Rabbit lgG | 100 slides | ￥7680.0 | |
| TLK0603-200R | Anti-Rabbit lgG | 200 slides | ￥13680.0 | |
| TLK0603-200C | Anti-Mouse and Rabbit lgG | 200 slides | ￥13680.0 |
5-Plex Tyramide Lumo Kit | TLK0503-50R
| 50 slides |
Goat Anti-Rabbit IgG
￥4080.0
5-Plex Tyramide Lumo Kit | TLK0503-50C
| 50 slides |
Goat Anti-Rabbit and Mouse IgG
￥4080.0
5-Plex Tyramide Lumo Kit | TLK0603-100R
| 100 slides |
Goat Anti-Rabbit IgG
￥7680.0
5-Plex Tyramide Lumo Kit | TLK0603-100C
| 100 slides |
Goat Anti-Rabbit and Mouse IgG
￥7680.0
5-Plex Tyramide Lumo Kit | TLK0603-200R
| 200 slides |
Goat Anti-Rabbit IgG
￥13680.0
5-Plex Tyramide Lumo Kit | TLK0603-200C
| 200 slides |
Goat Anti-Rabbit and Mouse IgG
￥13680.0
概述
酪胺信号放大系统 (Tyramide Signal Amplification, TSA) 是一种用于检测低丰度靶 标的信号放大技术。TSA 技术可将检测灵敏度提高 50-100 倍。MSBIO 将高性能荧光染料 MaxFluor 和 MaxSulf 系列与 TSA 技术结合，推出高灵敏度的酪胺信号放大系统 Tyramide Lumo Kit，帮助您实现对低丰度靶标的可靠检测与成像。
偶联有辣根过氧化物酶 HRP 的二抗与一抗结合后，将邻近区域的荧光染料分子的酪胺基团活化；活化后的染料分子可与靶标分子及靶标分子周围的芳香类氨基酸残基 (如酪氨酸、色氨酸、苯丙氨酸等) 共价结合并聚集沉积，在靶标分子处形成高密度荧光信号区域，从而将检测信号放大，实现对低丰度靶标分子的检测与成像。TSA 技术可在免疫组织化学（IHC）、免疫细胞化学（ICC）、荧光原位杂交 (FISH) 等多个应用场景中实现荧光信号放大。
成分
| 成分 | 规格 | 浓度 | 存储条件 |
|---|---|---|---|
| HRP Blocking Buffer | 40 mL | 1 × | 2 to 8°C |
| Antibody Dilution & Blocking Buffer | 40 mL | 1 × | 2 to 8°C |
| Tyramide Lumo Buffer | 40 mL | 1 × | 2 to 8°C |
| HRP polymer-conjugated Secondary Antibody (Anti-Mouse/Rabbit) |
20 mL | N/A | 2 to 8°C |
| Tyramide Dye | <25 μL per vial (50 slides) | 200 × | 2 to 8°C for 6 months; -20°C up to 12 months. |
| 50 μL per tube (100 slides) | |||
| 100 μL per tube (200 slides) | |||
| DAPI | 10 mL | 1 × | 2 to 8°C |
图集
光谱
Select your fluorescent dye to assess spectral compatibility:
系列产品
| Table 1. Single Fluorescence | 单色荧光系列 | |||||||
|---|---|---|---|---|---|---|---|
| Series 系列 |
Tyramide Dye 酪胺染料 |
Ex/Em (nm) | Color 颜色 |
HRP-Conjugate Secondary antibody HRP 偶联二抗 |
Cat# 货号 |
Size 规格 |
Price (¥) 价格 |
| Tyramide Lumo Kit (1x) 酪胺信号放大试剂盒（1x） |
MaxFluor 485 | 439/485 | Goat Anti-Mouse and Anti-Rabbit IgG | TLK0101-50C | 50 slides | 1380.00 | |
| MaxFluor 525 | 490/525 | TLK0102-50C | |||||
| MaxFluor 555 | 534/555 | TLK0103-50C | |||||
| MaxFluor 605 | 578/605 | TLK0104-50C | |||||
| MaxFluor 675 | 650/675 | TLK0105-50C | |||||
| MaxFluor 705 | 679/705 | TLK0106-50C | |||||
| MaxFluor 715 | 691/715 | TLK0107-50C | |||||
| MaxSulf 565 | 554/565 | TLK0201-50C | |||||
| MaxSulf 665 | 647/665 | TLK0202-50C | |||||
| MaxSulf 775 | 756/775 | TLK0203-50C | |||||
| Tyramide Lumo Kit (1x) 酪胺信号放大试剂盒（1x） |
MaxFluor 485 | 439/485 | Goat Anti-Rabbit IgG | TLK0101-50R | 50 slides | 1380.00 | |
| MaxFluor 525 | 490/525 | TLK0102-50R | |||||
| MaxFluor 555 | 534/555 | TLK0103-50R | |||||
| MaxFluor 605 | 578/605 | TLK0104-50R | |||||
| MaxFluor 675 | 650/675 | TLK0105-50R | |||||
| MaxFluor 705 | 679/705 | TLK0106-50R | |||||
| MaxFluor 715 | 691/715 | TLK0107-50R | |||||
| MaxSulf 565 | 554/565 | TLK0201-50R | |||||
| MaxSulf 665 | 647/665 | TLK0202-50R | |||||
| MaxSulf 775 | 756/775 | TLK0203-50R |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒（200×） |
MaxFluor 485 | 439/485 | Goat Anti-Mouse and Anti-Rabbit IgG | TLK0301-100C | 100 slides | 2480.00 | |
| MaxFluor 525 | 490/525 | TLK0302-100C | |||||
| MaxFluor 555 | 534/555 | TLK0303-100C | |||||
| MaxFluor 605 | 578/605 | TLK0304-100C | |||||
| MaxFluor 675 | 650/675 | TLK0305-100C | |||||
| MaxFluor 705 | 679/705 | TLK0306-100C | |||||
| MaxFluor 715 | 691/715 | TLK0307-100C | |||||
| MaxSulf 565 | 554/565 | TLK0401-100C | |||||
| MaxSulf 665 | 647/665 | TLK0402-100C | |||||
| MaxSulf 775 | 756/775 | TLK0403-100C |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒（200×） |
MaxFluor 485 | 439/485 | Goat Anti-Rabbit IgG | TLK0301-100R | 100 slides | 2480.00 | |
| MaxFluor 525 | 490/525 | TLK0302-100R | |||||
| MaxFluor 555 | 534/555 | TLK0303-100R | |||||
| MaxFluor 605 | 578/605 | TLK0304-100R | |||||
| MaxFluor 675 | 650/675 | TLK0305-100R | |||||
| MaxFluor 705 | 679/705 | TLK0306-100R | |||||
| MaxFluor 715 | 691/715 | TLK0307-100R | |||||
| MaxSulf 565 | 554/565 | TLK0401-100R | |||||
| MaxSulf 665 | 647/665 | TLK0402-100R | |||||
| MaxSulf 775 | 756/775 | TLK0403-100R |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒（200×） |
MaxFluor 485 | 439/485 | Goat Anti-Mouse and Anti-Rabbit IgG | TLK0301-200C | 200 slides | 4280.00 | |
| MaxFluor 525 | 490/525 | TLK0302-200C | |||||
| MaxFluor 555 | 534/555 | TLK0303-200C | |||||
| MaxFluor 605 | 578/605 | TLK0304-200C | |||||
| MaxFluor 675 | 650/675 | TLK0305-200C | |||||
| MaxFluor 705 | 679/705 | TLK0306-200C | |||||
| MaxFluor 715 | 691/715 | TLK0307-200C | |||||
| MaxSulf 565 | 554/565 | TLK0401-200C | |||||
| MaxSulf 665 | 647/665 | TLK0402-200C | |||||
| MaxSulf 775 | 756/775 | TLK0403-200C |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒（200×） |
MaxFluor 485 | 439/485 | Goat Anti-Rabbit IgG | TLK0301-200C | 200 slides | 4280.00 | |
| MaxFluor 525 | 490/525 | TLK0302-200R | |||||
| MaxFluor 555 | 534/555 | TLK0303-200R | |||||
| MaxFluor 605 | 578/605 | TLK0304-200R | |||||
| MaxFluor 675 | 650/675 | TLK0305-200R | |||||
| MaxFluor 705 | 679/705 | TLK0306-200R | |||||
| MaxFluor 715 | 691/715 | TLK0307-200R | |||||
| MaxSulf 565 | 554/565 | TLK0401-200R | |||||
| MaxSulf 665 | 647/665 | TLK0402-200R | |||||
| MaxSulf 775 | 756/775 | TLK0403-200R |
| Table 2. Multiplex Fluorescence | 多重荧光系列 | ||||||
|---|---|---|---|---|---|---|
| Series 系列 |
Tyramide Dye 酪胺染料 |
Color 颜色 |
HRP-Conjugate Secondary Antibody HRP 偶联二抗 |
Cat# 货号 |
Size 规格 |
Price (¥) 价格 |
| Tyramide Lumo Kit (1x) 酪胺信号放大试剂盒 (1x) |
3-Plex Tyramide Lumo Kit | Goat Anti-Mouse and Anti-Rabbit IgG | TLK0501-50C | 50 slides | 2180.00 | |
| 4-Plex Tyramide Lumo Kit | TLK0502-50C | 2780.00 | ||||
| 5-Plex Tyramide Lumo Kit | TLK0503-50C | 4080.00 | ||||
| 6-Plex Tyramide Lumo Kit | TLK0504-50C | 5380.00 | ||||
| 7-Plex Tyramide Lumo Kit | TLK0505-50C | 7280.00 | ||||
| Tyramide Lumo Kit (1x) 酪胺信号放大试剂盒 (1x) |
3-Plex Tyramide Lumo Kit | Goat Anti-Rabbit IgG | TLK0501-50R | 50 slides | 2180.00 | |
| 4-Plex Tyramide Lumo Kit | TLK0502-50R | 2780.00 | ||||
| 5-Plex Tyramide Lumo Kit | TLK0503-50R | 4080.00 | ||||
| 6-Plex Tyramide Lumo Kit | TLK0504-50R | 5380.00 | ||||
| 7-Plex Tyramide Lumo Kit | TLK0505-50R | 7280.00 |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒 (200×) |
3-Plex Tyramide Lumo Kit | Goat Anti-Mouse and Anti-Rabbit IgG | TLK0601-100C | 100 slides | 3980.00 | |
| 4-Plex Tyramide Lumo Kit | TLK0602-100C | 4980.00 | ||||
| 5-Plex Tyramide Lumo Kit | TLK0603-100C | 7680.00 | ||||
| 6-Plex Tyramide Lumo Kit | TLK0604-100C | 9980.00 | ||||
| 7-Plex Tyramide Lumo Kit | TLK0605-100C | 13580.00 |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒 (200×) |
3-Plex Tyramide Lumo Kit | Goat Anti-Rabbit IgG | TLK0601-100R | 100 slides | 3980.00 | |
| 4-Plex Tyramide Lumo Kit | TLK0602-100R | 4980.00 | ||||
| 5-Plex Tyramide Lumo Kit | TLK0603-100R | 7680.00 | ||||
| 6-Plex Tyramide Lumo Kit | TLK0604-100R | 9980.00 | ||||
| 7-Plex Tyramide Lumo Kit | TLK0605-100R | 13580.00 |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒 (200×) |
3-Plex Tyramide Lumo Kit | Goat Anti-Mouse and Anti-Rabbit IgG | TLK0601-200C | 200 slides | 7280.00 | |
| 4-Plex Tyramide Lumo Kit | TLK0602-200C | 9080.00 | ||||
| 5-Plex Tyramide Lumo Kit | TLK0603-200C | 13680.00 | ||||
| 6-Plex Tyramide Lumo Kit | TLK0604-200C | 18280.00 | ||||
| 7-Plex Tyramide Lumo Kit | TLK0605-200C | 24680.00 |
| Tyramide Lumo Kit (200×) 酪胺信号放大试剂盒 (200×) |
3-Plex Tyramide Lumo Kit | Goat Anti-Rabbit IgG | TLK0601-200R | 200 slides | 7280.00 | |
| 4-Plex Tyramide Lumo Kit | TLK0602-200R | 9080.00 | ||||
| 5-Plex Tyramide Lumo Kit | TLK0603-200R | 13680.00 | ||||
| 6-Plex Tyramide Lumo Kit | TLK0604-200R | 18280.00 | ||||
| 7-Plex Tyramide Lumo Kit | TLK0605-200R | 24680.00 |
| Table 3. Tyramide Dye | 酪胺染料系列 | |||||
|---|---|---|---|---|---|
| Tyramide Lumo 酪胺染料 |
Cat# 货号 |
Ex/Em (nm) | Size 规格 |
Price (¥) 价格 |
Color 颜色 |
| MaxFluor 485 Tyramide | FLD0101 | 439/485 | 200 Slides | 2580.00 | |
| MaxFluor 525 Tyramide | FLD0102 | 490/525 | |||
| MaxFluor 555 Tyramide | FLD0103 | 534/555 | |||
| MaxFluor 605 Tyramide | FLD0104 | 578/605 | |||
| MaxFluor 675 Tyramide | FLD0105 | 650/675 | |||
| MaxFluor 705 Tyramide | FLD0106 | 679/705 | |||
| MaxFluor 715 Tyramide | FLD0107 | 691/715 | |||
| MaxSulf 565 Tyramide | FLD0201 | 554/565 | |||
| MaxSulf 665 Tyramide | FLD0202 | 647/665 | |||
| MaxSulf 775 Tyramide | FLD0203 | 756/775 | |||
有关更多产品信息，请查看下方文档中的说明书附录
文档
疑难解答
| 问题 | 解决 |
|---|---|
| 信号强度过高 | 缩短酪胺染料染色时间。 |
| 减少一抗用量或缩短一抗孵育时间。 | |
| 信号强度过低 | 延长酪胺染料染色时间。 |
| 增加一抗用量或延长孵育时间。 | |
| 更换抗原修复液以增强抗原表位的暴露。 | |
| 背景荧光信号过高 | 更换细胞固定液。 |
| 延长内源性过氧化物酶淬灭时间。 | |
| 延长非特异性位点封闭时间。 | |
| 增加细胞或组织润洗次数和时间，或换用 PBST 润洗。 | |
| 减少二抗用量或缩短二抗孵育时间。 | |
| 无靶标信号或靶标信号弱 | 配制染料时，需充分混匀Tramide Lumo Buffer使用。 |
| 若1× Tramide Dye 长期存放在2-8°C，有可能会失效。 |